| IL-13

Interleukin 13 (IL-13) is a protein that is encoded by the IL13 gene.IL-13 was first cloned in 1993 and is located on chromosome 5q31.1 with a length of 1.4kb.It has a mass of 13 kDa and folds into 4 alpha helical bundles.The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling.IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes.Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	2.59	1.95	1.46	0.94	0.63	0.43	0.32	0.1
Corrected OD	2.49	1.85	1.36	0.84	0.53	0.33	0.22	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IL-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	91-105	102
Cell Culture Supernates(n=8)	94-108	105

 

| Linearity

Samples were spiked with high concentrations of Human IL-13 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
	Average (%)	90	93	96
1 : 4	Range (%)	88-103	87-108	105-115
	Average (%)	93	98	108